Lab Report on Enzyme Reactions

Lab report April 14, 2013 face-lift In this article, we will experiment on the evidential in authority of the enzyme by using three different strain tubes and measuring the come in of product they give off. To happen this we are difference to block out the sum of money of twine absorbance by using a special tool to serve us empathise our results. We will reveal how our end results show the event of the tot of concentration we apply to each establish tube. The results would be shown by the support of two graphs. IntroductionEnzymes are highly important because of the significant role they play in living things and animals. They are a doer which allows whether or not a reaction occurs. To find extinct the amount of strength an enzyme has, we need to first run into our outcome. In this experiment, our products will fixate the strength of our enzymes by measuring the amount of color change. We must understand the measurement of the appearing product means in the exp eriment. We will determine this by using a special equipment to help us understand our result better.In this study we will see how a specific enzyme could modify based on the different types of factors that chance on it. The question we are going to be addressing in this study is, will the amount of enzyme concentration affect a reaction? Materials and Methods The materials used in the making of this experiment are as stated Three test tubes, 10. 5mL of distilled water, 6mL of Catechol, 1. 5mL of Extract(potato), a Spectronic 20 (spectrophotometer), turn out pencil, a Test tube rack, three Parafilm, tissues to clean the Spectronic 20 and the test tubes, 3 pipets and a timer.So we wont confuse ourselves, we used the develop pencil to label each test tube as we place the postulate amount of substance in each of them. To avoid all slight errors, we besides used three different pipets for the three required materials, which are the distilled water, catechol and the extract, resp ectively. Its important to take the precise measurement so there wont be any errors in doing the experiment. Its also important that the extract goes last due to the reaction occurring in a timed pace. ResultsAccording to the graph shown above, we see our lay out of the experiment macrocosm done. We can see how the materials are being distributed in each test tube according to the amount of ingredients required. This is highly significant because a slight mistake can impact the experiment being done. Our end result is as follows According to the experiment done today, we see that in test tube two, it show the most absorbance or color change. This shows that the enzymes strength was stronger than the rest, resulting into having more reaction and color change among the new(prenominal)s.Discussion As stated before, the question we had was, will the amount of enzyme concentration affect a reaction? The end result was yes, the amount of enzyme concentrated inserted into each test tube did have a significant affect to the reaction by the amount of products it gave off (color strength). We used the extract (enzyme) as our independent versatile because this is what we are testing. In contrast, we used the amount of absorbance as our dependent variable.We also made sure that our control done in this experiment was the catechol. As predicted, we were able to have more enzyme reaction in one of the test tubes than the others. The knowledge we gain today was how much the amount of concentration enzyme affects the other ingredients. We see that our experiment done today can further widen into more research as in how enzymes are used to quick an action. Its also important because we should know the reason why and how enzymes reactions occur at a fast rate.